This guide contains information, strategies and resources to help HIV programmers identify and meet the needs of women and girls in all their diversity. It contains tools, evidence and good practice to ensure that HIV programming responds to and addresses harmful gender norms, structures and stereotypes that act as a barrier to HIV prevention, treatment and care, and the realisation of sexual and reproductive health and rights. This guide supports a more nuanced understanding of gender-related barriers and how aspects of identities intersect with HIV, gender norms, sexual and reproductive health and rights and access to health services.